Human CellExp TNFR1 / CD120a / TNFRSF1A, human recombinant protein
TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P19438 |
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Calculated MW | This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22 kDa. The predicted N-terminus is Ile 22. DTT-reduced Protein migrates as 30-35 kDa due to different glycosylation. |
Gene ID | 7132 |
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Gene Symbol | TNFRSF1A |
Other Names | TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60. |
Gene Source | Human |
Source | HEK293 cells |
Assay&Purity | SDS-PAGE; ≥92% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Measured by its binding ability in a functional ELISA. Immobilized human TNFα at 10 µg/mL (100 µl/well) can bind biotinylated human TNFRSF1A. The EC50 of biotinylated human TNFRSF1A is 0.02 µg/mL. |
Target/Specificity | TNFR1 / CD120a / TNFRSF1A |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Format | Lyophilized |
Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
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Background
Tumor necrosis factor receptor 1 (TNF-R1) also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial Hibernian fever (FHF).
References
Loetscher H.,et al.Cell 61:351-359(1990).
Schall T.J.,et al.Cell 61:361-370(1990).
Himmler A.,et al.DNA Cell Biol. 9:705-715(1990).
Nophar Y.,et al.EMBO J. 9:3269-3278(1990).
Gray P.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:7380-7384(1990).
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