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Human CellExp VTCN1, human recombinant protein
B7-H4, VTCN1, B7S1, B7h.5
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q7Z7D3 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26.1 kDa. The predicted N-terminus is Phe 29. DTT-reduced Protein migrates as 45-60 kDa due to glycosylation. |
| Gene ID | 79679 |
|---|---|
| Gene Symbol | VTCN1 |
| Other Names | B7-H4, VTCN1, B7S1, B7h.5 |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥90% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.75 - 3 µg/ml. |
| Target/Specificity | VTCN1 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
References
Sica G.L.,et al.Immunity 18:849-861(2003).
Zang X.,et al.Proc. Natl. Acad. Sci. U.S.A. 100:10388-10392(2003).
Sun Y.,et al.Lung Cancer 53:143-151(2006).
Mao Y.,et al.Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
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