Human CellExp ICOSLG /B7-H2 /CD275, human recombinant protein
ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand.
|Calculated MW||This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 27.5 kDa. The predicted N-terminus is Asp 19. DTT-reduced Protein migrates as 45-50 kDa due to glycosylation.|
|Other Names||ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand.|
|Results||Measured by its binding ability in a functional ELISA. Immobilized human ICOSLG / B7H2 at 1 µg/ml (100 µl/well) can bind human ICOS with a linear range of 3.2 - 400 ng/ml.|
|Target/Specificity||ICOSLG /B7-H2 /CD275|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
Wang S.,et al.Blood 96:2808-2813(2000).
Yoshinaga S.K.,et al.Int. Immunol. 12:1439-1447(2000).
Ling V.,et al.J. Immunol. 164:1653-1657(2000).
Ling V.,et al.Patent number WO0121796, 29-MAR-2001.
Ishikawa K.,et al.DNA Res. 5:169-176(1998).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.