Human CellExp CD58 /LFA-3, human recombinant protein
CD58, LFA3, Ag3
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P19256 |
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Calculated MW | This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22.3 kDa. The predicted N-terminus is Phe 29. DTT-reduced Protein migrates as 30-45 kDa due to different glycosylation. |
Gene ID | 965 |
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Gene Symbol | CD58 |
Other Names | CD58, LFA3, Ag3 |
Gene Source | Human |
Source | HEK293 cells |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Measured in a cell proliferation assay using human CD3+ T cells in the presence of anti-CD3. The ED50 for this effect is typically 1 - 5 µg/ml in the presence of anti-CD3 immobilized at 25 ng/ml. |
Target/Specificity | CD58 /LFA-3 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Format | Lyophilized |
Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.
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