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Human CellExp CD62E/E-Selectin, human recombinant protein
SELE, RP1-117P20.2, CD62E, ELAM1, ESEL, LECAM2, E-Selectin
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P16581 |
|---|---|
| Calculated MW | This protein is fused with a 6×his tag at C-terminus and has a calculated MW of 60 kDa expressed. The predicted N-terminus is Trp22. Protein migrates as 110 kDa in reduced SDS-PAGE resulting from glycosylation. |
| Gene ID | 6401 |
|---|---|
| Gene Symbol | SELE |
| Other Names | SELE, RP1-117P20.2, CD62E, ELAM1, ESEL, LECAM2, E-Selectin |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10^4 cells/well are added to human E-Selectin coated plates (2 µg/mL with 100 µL/well), >80% cells will adhere after 1 hour incubation at 37°C. |
| Target/Specificity | CD62E/E-Selectin |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
E-Selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1β and TNFα. E selectin has a cassette structure: an N-terminal, C-type lectin domain, an EGF (epidermal-growth-factor)-like domain, 6 Sushi domain (SCR repeat) units, a transmembrane domain (TM) and an intracellular cytoplasmic tail (cyto). During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The local release of cytokines IL-1 and TNF-α by damaged cells induces the over-expression of E-selectin on endothelial cells of nearby blood vessels. E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.
References
Hession C.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:1673-1677(1990).
Bevilacqua M.P.,et al.Science 243:1160-1165(1989).
Collins T.,et al.J. Biol. Chem. 266:2466-2473(1991).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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