|Calculated MW||This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 21.6 kDa. The predicted N-terminus is Ala 22. DTT-reduced Protein migrates as 26-33 kDa due to glycosylation.|
|Other Names||Basigin, BSG, 5F7, CD147, EMMPRIN, M6, OK, TCSF.|
|Results||Measured by the ability of the immobilized protein to induce active MMP1 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 0.5-4 µg/ml.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
CD147, also known as Basigin (BSG), or extracellular matrix metalloproteinase inducer (EMMPRIN). The human basigin protein contains 269 amino acids that form two heavily glycosylated C2 type immunoglobulin-like domains at the N-terminal extracellular portion. A second form of basigin has also been characterized that contains one additional immunoglobulin-like domain in its extracellular portion. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition and regulate several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.
Kasinrerk W.,et al.J. Immunol. 149:847-854(1992).
Miyauchi T.,et al.J. Biochem. 110:770-774(1991).
Biswas C.,et al.Cancer Res. 55:434-439(1995).
Wakasugi H.,et al.Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
Decastro R.,et al.Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
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