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Human CellExp GFRA2 /GDNFRB, human recombinant protein
GFRA2, GDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | O00451 |
|---|---|
| Calculated MW | This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 47.6 kDa. The predicted N-terminus is Ser 22. DTT-reduced Protein migrates as 60-70 KDa due to glycosylation. |
| Gene ID | 2675 |
|---|---|
| Gene Symbol | GFRA2 |
| Other Names | GFRA2, GDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2. |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Neurturin at 1 µg/ml can bind rhGFRA2 with an apparent KD < 8 nM. |
| Target/Specificity | GFRA2 /GDNFRB |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
GDNF family receptor alpha-2 (GFRA2) also known as GDNF receptor beta, Neurturin receptor alpha, RET ligand 2, TGF-beta-related neurotrophic factor receptor 2, is a cell membrane protein which belongs to the GDNFR family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF-beta superfamily of growth factors. Three receptors for these factors, GFRα-1, GFRα-2 and GFRα-3 have been identified. The isoform 1 of GFRA2 is found in both brain and placenta. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor and also able to mediate GDNF signaling through the RET tyrosine kinase receptor. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It can also mediate GDNF signaling through the RET tyrosine kinase receptor.
References
Baloh R.H.,et al.Neuron 18:793-802(1997).
Suvanto P.,et al.Hum. Mol. Genet. 6:1267-1273(1997).
Sanicola M.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:6238-6243(1997).
Yoong L.F.,et al.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
Nusbaum C.,et al.Nature 439:331-335(2006).
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