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Human CellExp Carbonic Anhydrase 4/CA4, human recombinant protein
CA4, Carbonic anhydrase 4
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P22748 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 31 kDa. The predicted N-terminus is Ala 19. DTT-reduced Protein migrates as 33 kDa in SDS-PAGE. |
| Gene ID | 762 |
|---|---|
| Gene Symbol | CA4 |
| Other Names | CA4, Carbonic anhydrase 4 |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥92% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its esterase activity. The specific activity is >2 pmol/min/ µg. |
| Target/Specificity | Carbonic Anhydrase 4/CA4 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Carbonic anhydrase 4 (CA4) is also known as Carbonate dehydratase IV, Carbonic anhydrase IV, which belongs to the alpha-carbonic anhydrase family. CA4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. The enzyme regulation is activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. CA4 can interact with SLC4A4.
References
Okuyama T.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:1315-1319(1992).
Okuyama T.,et al.Genomics 16:678-684(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Zody M.C.,et al.Nature 440:1045-1049(2006).
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