Human CellExp Carbonic Anhydrase 10/CA10, human recombinant protein
CA10, Carbonic Anhydrase X, CARPX, hucep-15
|Calculated MW||This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 32.6 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 37 kDa due to glycosylation.|
|Other Names||CA10, Carbonic Anhydrase X, CARPX, hucep-15|
|Results||Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 µg rh CA10 / CARPX enzyme at 100 ng/ µl in 100 µL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/ µg.|
|Target/Specificity||Carbonic Anhydrase 10/CA10|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
Carbonic anhydrase-related protein 10 (CA10) is also known as Carbonic anhydrase-related protein X (CARP X or CARPX), Cerebral protein 15 (hucep-15), which belongs to the alpha-carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. CA10 / CARPX is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development.
Okamoto N.,et al.Biochim. Biophys. Acta 1518:311-316(2001).
Hewett-Emmett D.,et al.Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
Yoshimoto M.,et al.Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
Kleiderlein J.J.,et al.Hum. Genet. 103:666-673(1998).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
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