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Human CellExp Carbonic Anhydrase 10/CA10, human recombinant protein
CA10, Carbonic Anhydrase X, CARPX, hucep-15
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9NS85 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 32.6 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 37 kDa due to glycosylation. |
| Gene ID | 56934 |
|---|---|
| Gene Symbol | CA10 |
| Other Names | CA10, Carbonic Anhydrase X, CARPX, hucep-15 |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 µg rh CA10 / CARPX enzyme at 100 ng/ µl in 100 µL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/ µg. |
| Target/Specificity | Carbonic Anhydrase 10/CA10 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Carbonic anhydrase-related protein 10 (CA10) is also known as Carbonic anhydrase-related protein X (CARP X or CARPX), Cerebral protein 15 (hucep-15), which belongs to the alpha-carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. CA10 / CARPX is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development.
References
Okamoto N.,et al.Biochim. Biophys. Acta 1518:311-316(2001).
Hewett-Emmett D.,et al.Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
Yoshimoto M.,et al.Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
Kleiderlein J.J.,et al.Hum. Genet. 103:666-673(1998).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
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