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Human CellExp IGF-1, human recombinant protein
IGF-I, IGF1A, somatomedin C, MGF
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P05019 |
|---|---|
| Calculated MW | This protein rhIGFI-Fc, fused with Fc fragment of human IgG1 at the N-terminus, has a calculated MW of 35 kDa. DTT-reduced Protein migrates as 35 kDa. |
| Gene ID | 3479 |
|---|---|
| Gene Symbol | IGF1 |
| Other Names | IGF-I, IGF1A, somatomedin C, MGF |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥98% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | The ED50 for this effect is typically 0.5-2.5 ng/mL. |
| Target/Specificity | IGF-1 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Insulin-like growth factor 1 (IGF-1) also known as somatomedin C, IGF1A, IGFI, sulfation factor, and is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. IGF-1 binds to at least two cell surface receptors: the Insulin-like growth factor 1 receptor, abbreviated as "IGF1R", and the insulin receptor. The IGF-1 receptor seems to be the "physiologic" receptor - it binds IGF-1 at significantly higher affinity than the IGF-1 that is bound to the insulin receptor. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. Its primary action is mediated by binding to its specific receptor IGF1R, present on many cell types in many tissues. Binding to the IGF1R, a receptor tyrosine kinase, initiates intracellular signaling; IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and proliferation, and a potent inhibitor of programmed cell death. Insulin-like growth factor 1 has been shown to bind and interact with all the IGF-1 Binding Proteins (IGFBPs), of which there are six (IGFBP1-6).
References
Jansen M.,et al.Nature 306:609-611(1983).
de Pagter-Holthuizen P.,et al.FEBS Lett. 195:179-184(1986).
le Bouc Y.,et al.FEBS Lett. 196:108-112(1986).
Rotwein P.,et al.J. Biol. Chem. 261:4828-4832(1986).
Rotwein P.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:77-81(1986).
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