|Calculated MW||38.2 kDa (18-339 aa + N-terminal poly-his tag)|
|Other Names||CTSB, CPSB|
|Results||> 5000 mU/mg (1 U = 1 µmole/min, after complete activation)|
|Application Notes||Reconstitute in de-ionized water to 1 mg/ml and store at –20°C. Avoid repeated freezing and thawing cycles.|
|Storage||-20°C; Lyophilized from a proprietary buffer.|
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Provided below are standard protocols that you may find useful for product applications.
Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. Biovision’s Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.
Chan S.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:7721-7725(1986).
Cao L.,et al.Gene 139:163-169(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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