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Procathepsin B, Human recombinant protein
CTSB, CPSB
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P07858 |
|---|---|
| Calculated MW | 38.2 kDa (18-339 aa + N-terminal poly-his tag) |
| Gene ID | 1508 |
|---|---|
| Gene Symbol | CTSB |
| Other Names | CTSB, CPSB |
| Gene Source | Human |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Results | > 5000 mU/mg (1 U = 1 µmole/min, after complete activation) |
| Target/Specificity | Procathepsin B |
| Application Notes | Reconstitute in de-ionized water to 1 mg/ml and store at –20°C. Avoid repeated freezing and thawing cycles. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from a proprietary buffer. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. Biovision’s Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.
References
Chan S.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:7721-7725(1986).
Cao L.,et al.Gene 139:163-169(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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