|Calculated MW||63.0 kDa|
|Other Names||RAF, RAF proto-oncogene serine/threonine-protein kinase , Proto-oncogene c-RAF|
|Source||Baculovirus (Sf9 insect cells)|
|Storage||-80°C; Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).
Bonner T.I.,et al.Nucleic Acids Res. 14:1009-1015(1986).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Bonner T.I.,et al.Mol. Cell. Biol. 5:1400-1407(1985).
Andreu-Perez P.,et al.Sci. Signal. 4:RA58-RA58(2011).
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