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Pim 1, Active recombinant protein
Pim, Serine/threonine-protein kinase Pim-1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P11309 |
|---|---|
| Concentration | 0.1 |
| Calculated MW | 62.0 kDa |
| Gene ID | 5292 |
|---|---|
| Gene Symbol | PIM1 |
| Other Names | Pim, Serine/threonine-protein kinase Pim-1 |
| Source | Baculovirus (Sf9 insect cells) |
| Assay&Purity | SDS-PAGE; ≥90% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Format | Liquid |
| Storage | -80°C; Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol). |
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Background
The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Accumulating data support that the expression of Pim1 is mediated through activation of the JAK/STAT pathway. Some of the substrates of Pim1 include p21 Cip1, nuclear mitotic appartus protein, PTP-U2S and Socs-1. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis. Pim1 interacts with c-Myb via the DNA binding domain and regulates its transcriptional activity.
References
Reeves R.,et al.Gene 90:303-307(1990).
Zakut-Houri R.,et al.Gene 54:105-111(1987).
Domen J.,et al.Oncogene Res. 1:103-112(1987).
Meeker T.C.,et al.J. Cell. Biochem. 35:105-112(1987).
Xie Y.,et al.Oncogene 25:70-78(2006).
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