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ZAP70, Active recombinant protein

ZAP70, zeta-chain (TCR) associated protein kinase 70kDa

Product info
Primary Accession P43403
Concentration 0.1
Calculated MW 96.0 kDa
Additional Info
Gene ID 7535
Gene Symbol ZAP70
Other Names ZAP70, zeta-chain (TCR) associated protein kinase 70kDa, Syk-related tyrosine kinase
Source Baculovirus (Sf9 insect cells)
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 HPLC;
Recombinant Yes
Format Liquid
Storage -80°C; Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Citations (0)

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ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. ZAP70 tyrosine kinase is tyrosine phosphorylated in Jurkat T cells and in purified peripheral T cells after MHC-I ligation. The phosphorylation of ZAP70 after MHC-I ligation is dependent on TCR/CD3 surface expression. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2). Another substrate of ZAP70 is LAT (linker for activation of T cells). Direct tyrosine phosphorylation of LAT with activated protein-tyrosine kinase Zap70 is necessary and sufficient for the association and activation of signalling proteins. Zap-70 efficiently phosphorylates LAT on tyrosine residues at positions 226, 191, 171, 132 and 127. By substituting these tyrosine residues in LAT with phenylalanine and by utilizing phosphorylated peptides derived from these sites, the tyrosine residues in LAT have been shown to be required for the direct interaction and activation of Vav, p85/p110α and phospholipase Cγ1 (PLCγ1) (3).


Chan A.C.,et al.Cell 71:649-662(1992).
Kuroyama H.,et al.Biochem. Biophys. Res. Commun. 315:935-941(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Arpaia E.,et al.Cell 76:947-958(1994).
Isakov N.,et al.J. Exp. Med. 181:375-380(1995).

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$ 295.00
Cat# PBV11327r
(40 western blots)
Availability: 5-7days
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