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DUSP21, human recombinant protein

Dual specificity protein phosphatase 21

     
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Product info
Primary Accession Q9H596
Concentration 0.25
Calculated MW 24.1 kDa (214 aa, 1-190 aa + His Tag)
Additional Info
Gene ID 63904
Gene Symbol DUSP21
Other Names Dual specificity protein phosphatase 21
Gene Source Human
Source E.coli
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity is > 1800 unit/mg
Sequence MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM
Target/Specificity DUSP21
Format Liquid
Storage -20°C; 0.25 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 200 mM NaCl and 2 mM DTT
Citations (0)

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Background

DuSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DuSP21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

References

Hood K.L.,et al.Biochem. Biophys. Res. Commun. 298:545-551(2002).
Mao Y.,et al.Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ross M.T.,et al.Nature 434:325-337(2005).
Sjoeblom T.,et al.Science 314:268-274(2006).

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$ 375.00
Cat# PBV11375r-50
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