|Calculated MW||24.1 kDa (214 aa, 1-190 aa + His Tag)|
|Other Names||Dual specificity protein phosphatase 21|
|Results||Specific activity is > 1800 unit/mg|
|Sequence||MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM|
|Storage||-20°C; 0.25 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 200 mM NaCl and 2 mM DTT|
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Provided below are standard protocols that you may find useful for product applications.
DuSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DuSP21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Hood K.L.,et al.Biochem. Biophys. Res. Commun. 298:545-551(2002).
Mao Y.,et al.Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ross M.T.,et al.Nature 434:325-337(2005).
Sjoeblom T.,et al.Science 314:268-274(2006).
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