|Calculated MW||65.3 kDa (578 aa, 1-558 aa + His Tag)|
|Other Names||Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36.|
|Results||Specific activity is > 90 units/mg|
|Sequence||MGSSHHHHHH SSGLVPRGSH MAALTRDPQF KLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK FCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT LEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ|
|Storage||-20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1 mM DTT|
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Provided below are standard protocols that you may find useful for product applications.
Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Gurney M.E.,et al.Submitted (MAR-1987) to the EMBL/GenBank/DDBJ databases.
Yakirevich E.,et al.Biol. Reprod. 62:1016-1023(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Walker J.I.H.,et al.Genomics 7:638-643(1990).
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