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PRPS1, human recombinant protein

Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1

     
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Product info
Primary Accession P60891
Concentration 1
Calculated MW 36.9 kDa (338 aa, 1-318 aa + His Tag)
Additional Info
Gene ID 5631
Gene Symbol PRPS1
Other Names Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1
Gene Source Human
Source E.coli
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity: > 1 Units/ml
Sequence MGSSHHHHHH SSGLVPRGSH MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI RRTHNGESVS YLFSHVPL
Target/Specificity PRPS1
Format Liquid
Storage -20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT and 0.1 M NaCl
Citations (0)

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Background

PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

References

Roessler B.J.,et al.Nucleic Acids Res. 18:193-193(1990).
Sonoda T.,et al.J. Biochem. 109:361-364(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ross M.T.,et al.Nature 434:325-337(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 285.00
Cat# PBV11387r-100
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