PRPS1, human recombinant protein
Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P60891 |
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Concentration | 1 |
Calculated MW | 36.9 kDa (338 aa, 1-318 aa + His Tag) |
Gene ID | 5631 |
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Gene Symbol | PRPS1 |
Other Names | Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1 |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Specific activity: > 1 Units/ml |
Sequence | MGSSHHHHHH SSGLVPRGSH MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI RRTHNGESVS YLFSHVPL |
Target/Specificity | PRPS1 |
Format | Liquid |
Storage | -20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT and 0.1 M NaCl |
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Background
PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
References
Roessler B.J.,et al.Nucleic Acids Res. 18:193-193(1990).
Sonoda T.,et al.J. Biochem. 109:361-364(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ross M.T.,et al.Nature 434:325-337(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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