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NFNB, bacterial recombinant protein

Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.

     
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Product info
Primary Accession A1A8L0
Concentration 1
Calculated MW 26.0 kDa (237 aa, 1-217 aa + His Tag)
Additional Info
Gene ID 4493867
Gene Symbol NFNB
Other Names Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.
Gene Source E. Coli
Source E.coli
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity: > 2 units/ml
Sequence MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV
Target/Specificity NFNB
Format Liquid
Storage -20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT and 50 mM NaCl
Citations (0)

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Background

NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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$ 295.00
Cat# PBV11389r
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