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Carbonic Anhydrase 3, human recombinant protein
CA3, Carbonic anhydrase 3
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P07451 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 30.4 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 28-30 kDa in SDS-PAGE. |
| Gene ID | 761 |
|---|---|
| Gene Symbol | CA3 |
| Other Names | CA3, Carbonic anhydrase 3 |
| Gene Source | Human |
| Source | E.coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | The specific activity is >1 pmol/min/ µg. |
| Target/Specificity | Carbonic Anhydrase 3 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Carbonic anhydrase 3 (CA3) is also known as Carbonate dehydratase III, Carbonic anhydrase III, which belongs to the alpha-carbonic anhydrase family. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The catalytic activity of carbonic anhydrase 3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.
References
Lloyd J.,et al.Gene 41:233-239(1986).
Wade R.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:9571-9575(1986).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lloyd J.,et al.Genes Dev. 1:594-602(1987).
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