Glycine Oxidase H244K, Bacillus subtilis recombinant protein
Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
|Calculated MW||43.1 kDa (1-369 aa, NT His Tag)|
|Other Names||Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO|
|Gene Source||Bacillus subtilis|
|Storage||-20°C; 4.0 mg/ml in 20 mM potassium phosphate, 100 mM NaCl, pH 8.0 and 10% glycerol|
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Provided below are standard protocols that you may find useful for product applications.
Glycine oxidase (GO) from Bacillus subtilis (EC 18.104.22.168) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.
Kunst F.,et al.Nature 390:249-256(1997).
Nishiya Y.,et al.FEBS Lett. 438:263-266(1998).
Job V.,et al.J. Biol. Chem. 277:6985-6993(2002).
Settembre E.C.,et al.Biochemistry 42:2971-2981(2003).
Moertl M.,et al.J. Biol. Chem. 279:29718-29727(2004).
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