Single-Stranded DNA Binding Protein
SSB
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Concentration | 5 µg/ µl |
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Calculated MW | Consists of four identical 18.9 kDa subunits |
Other Names | SSB |
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Source | E. coli strain containing an overproducing clone of E. coli SSB protein. |
Assay&Purity | SDS-PAGE;≥95% |
Recombinant | Yes |
Format | Liquid |
Storage | -20°C;Recombinant Single-Stranded DNA Binding Protein IN 50 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1.0 mM DTT, 50% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Single-Stranded DNA Binding Protein (SSB) binds with high affinity in a cooperative manner to single-stranded DNA and does not bind well to double-stranded DNA. After binding single-stranded DNA, SSB destabilizes helical duplexes, thereby allowing DNA polymerases to access their substrate more easily.
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