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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Ghost Dye™ Violet 450   

Ghost Dye™ Violet 450

     
  • FC - Ghost Dye™ Violet 450 RTB10001
    LEFT: Mouse thymocytes were incubated overnight at 4℃ (dashed) or 37℃ (solid) and stained with Ghost Violet 450. RIGHT: Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Violet 450. Viable gate is indicated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
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Citations (0)

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Background

Ghost Dye™ Violet 450 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Violet 450 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

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$ 44.00
$ 170.00
Cat# RTB10001
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