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Provided below are standard protocols that you may find useful for product applications.
Tonbo Biosciences Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains the following components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma.
- Transcription Factor Fix/Perm Concentrate (4X) (Cat. No. TNB-1020-L050): 50 mL. A concentrated solution which, when diluted with Transcription Factor Fix/Perm Diluent (1X) (cat. no. TNB-1022-L160), provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies.
- Transcription Factor Fix/Perm Diluent (1X) (Cat. No. TNB-1022-L160): 160 mL. Intended for use as a diluent for Transcription Factor Fix/Perm Concentrate (4X)
- Flow Cytometry Perm Buffer (10X) (Cat. No. TNB-1213-L150): 150 mL. Provided as a concentrate which, when diluted with distilled water to a 1X solution, provides best results in intracellular staining protocols for cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and wash steps.
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