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Validation Service

Antibody Validation Services

Let Us Be Your Lab

Certify that your antibodies work, while saving time and money. Abgent’s experienced staff custom validates more than 1,000 antibodies each month in Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC) and additional applications. Proper validation with controls and optimization ensure the quality of the antibody, and establish confidence with the researcher.

  1. 20,000+ antibodies successfully optimized over the past 15 years
  2. Access to 200+ tissues and cell lines to ensure validation is biologically relevant
  3. High-throughput and low-throughput capacities housed within the WuXi AppTec Research and Development Center in Suzhou, China
  4. Scalability – testing of up to 2,000 unique antibodies per month
  5. Flexibility using our in-house developed protocols or customer designed protocols and model systems
  6. Web-enabled SuperView software enables customer to track/control validation in real-time
  7. SOP controlled workflow with regular quality assurance audits
  8. Fast turnaround time and competitive pricing
  9. AAALAC and ISO accredited facilities

Abgent customers include academic and biotech labs, as well as the largest antibody companies in the world. We make validation easy and affordable for you. It is what we do, day in and day out, with excellence.

Let Us Be Your Lab

Order Information:

Phone:
USA: 888.735.7227 (toll free)
UK: +44 (0) 1628 639753

Email:
USA: quote@abgent.com
UK: eurosales@abgent.com

Fax:
USA: 858-622-0609
UK: +44 (0) 628 200753

SuperView

Abgent's Antibody Validation Management System "SuperView". Login from anywhere in the world and manage your custom antibody validation projects at your convenience.

Learn More
Immunofluorescence (IF)

Immunofluorescence (IF)

Fluorescent image of Hela cells stained with PPARA Antibody. This antibody was diluted at 1:25. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Immunohistochemical analysis (IHC)

ImmImmunohistochemistry (IHC)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using PPARA Antibody. This antibody was diluted at 1:25. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow Cytometric (FC)

Flow Cytometric (FC)

Flow cytometric analysis of Hela cells using PPARA Antibody (green), compared to a isotype control of mouse IgG1(blue). This antibody was diluted at 1:25. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Western Blot image (WB)

Western Blot (WB)

Western blot analysis of lysates from Hela, Jurkat, mouse NIH/3T3 cell line (from left to right), using PPARA Antibody. This antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates are loaded at 35ug per lane.

SuperView Abgent's Antibody Validation Management System "SuperView".
Login from anywhere in the world and manage your custom antibody validation projects at your convenience. Learn More

Abgent Antibody Validation Service